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Using standard CRISPR-based homologous recombination techniques in embryonic stem (ES) cells, CAG promoter-Frt-Stop-Frt followed by lox-Stop-lox and Casp3 expression cassette were inserted into the Gt(ROSA)26Sor locus. Chimeras were generated by blastocyst injection and subsequent germline transmission was confirmed by tail PCR. The neo-selection cassette was excised using a phiC31 strain. (J:341426)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
