A loxP site was inserted upstream of exon 1 and an FRT and loxP flanked neo cassette was inserted downstream of exon 2 via homologous recombination. Flp mediated recombination removed the neo cassette leaving exons 1 and 2 floxed. (J:301375)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count