A loxP site was inserted upstream of exon 1 and an FRT and loxP flanked neo cassette was inserted downstream of exon 1 via homologous recombination. Flp-mediated recombination removed the neo cassette leaving exon 1 floxed. (J:301375)
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A loxP site was inserted upstream of exon 1 and an FRT and loxP flanked neo cassette was inserted downstream of exon 1 via homologous recombination. Flp-mediated recombination removed the neo cassette leaving exon 1 floxed. (J:301375)