The targeting vector is designed to contain (from 5'; to 3') an FRT site, an En2 splice acceptor, an internal ribosome entry site (IRES) fused to an EGFP sequence with a polyadenylation signal, and a floxed cassette containing a neo and a second FRT site all upstream of exon 4. A third loxP was placed downstream of exon 4. The frt-flanked neomycin cassette was removed in the germline via FLP-mediated recombination. (J:340358)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
C57BL/6J
Targeted
Insertion
--
1
3
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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