Il10ra^em1Ccg

Threonine codon 86 (ATA) in exon 3 was changed to isoleucine (ACA) (p.T86I) using an sgRNA (targeting GGTGAACGTTGTGAGATCACAGG) and an ssODN template with CRISPR/Cas9 technology. In this allele an unintended 8 bp deletion (TCACAGGA GRCm39:chr9:g.45177878_45177885) caused a frameshift and premature stop codon (p.C83Hfs*33). (J:340910)