Igs2^{tm3(CAG-CaMBI300)Mzlin}

The allele is created using site-specific PhiC31 integrase-mediated transgenesis to insert a loxP-flanked stop cassette, and the Antares-derived, orange-emitting, calcium-modulated bioluminescent indicator (CaMBI) followed by a polyA signal and directed by a chicken beta-actin promoter with a CMV enhancer (CAG promoter). CamBI 300 is composed of CyOFP1::N-terminal NLuc::1aa linker (I)::CaM::1aa linker (G)::M13::2aa linker (EL)::C-terminal NLuc::CyOFP1. (J:341258)