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The allele is created using site-specific PhiC31 integrase-mediated transgenesis to insert a loxP-flanked stop cassette, a Kozak sequence and the CyOFP1-NanoLuc-CyOFP1 fusion bioluminescent reporter (Antares) directed by a chicken beta-actin promoter with a CMV enhancer (CAG promoter). Antares is composed of the highly-catalytic, blue-emitting firefly luciferase derivative NanoLuc fused to two copies of the fluorescent protein CyOFP1. CyOFP1 is a basic (constitutively fluorescent) long stokes shift fluorescent protein derived from Entacmaea quadricolor. (J:341167)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
