The targeting construct inserted a loxP site upstream of exon 48, GFP reporter gene at the STOP codon, and FRT-flanked neomycin resistance cassette with 3' loxP site downstream of the 3' UTR. FLP-mediated recombination removed the selection cassette. Cre-mediated recombination removed exon 48 and 49 along with the reporter gene. (J:340902)