Tlr4^em1Svog

Aspartic acid codon 298 (GAT) in exon 3 was changed to glycine (GGT) (p.298G) and asparagine codon 397 (AAC) in exon 3 to isoleucine (ATC) (p.N397I) using sgRNAs and ssODN templates with CRISPR/Cas9 technology. The mutations replicate the human p.D299G (c.896A>G, rs4986790) and p.T399I (c.1196C>T, rs4986791) mutations associated with LPS hyporesponsiveness and differential susceptibility to many infectious and non-infectious diseases. (J:303648)