The transgene contains linearized modified BAC DNA containing the Actb gene followed by a tetO-ChR2 cassette. FLP-mediated recombination removed the FRT-flanked neo selection cassette. (J:186771)
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The transgene contains linearized modified BAC DNA containing the Actb gene followed by a tetO-ChR2 cassette. FLP-mediated recombination removed the FRT-flanked neo selection cassette. (J:186771)