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CRISPR/cas9 genome editing is used to insert a human EEF1a1 promoter followed by::Lox2272::LoxP::EGFP (inverted)::P2A::APEX-NES (inverted)::FLAG tag::Lox2272::LoxP::hGH double floxed inverted orientation cassette into intron 1 of Gt(ROSA)26Sor. APEX2 is an engineered monomeric peroxidase reporter derived from dimeric soybean ascorbate peroxidase. (J:340416)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
