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Recombination-mediated cassette exchange (RMCE) inserted a cre-conditional FLEX (flip-excision) cassette with inserted a CAG promoter in front of the FLEX-cassette containing the coding sequence of hM4Di-mKateT was inserted into the FLEX cassette in reverse orientation to the promoter and a rabbit globulin polyA cassette including stop codons in every reading frame downstream of the FLEX cassette, in the same direction as hM4Di. The hM4D ORF is followed by a 6x His Tag and a V5 tag; however, these could not be detected by immunohistochemistry on PFA fixed tissue. (J:304133)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
