Chd2^em1Guof

Arginine codon 1684 (CGT) was changed to histidine (CAT) (c.5051G>A, p.R1684H) using an sgRNA (targeting CCCACCGTTCTGGGAGCTACCGC) and an ssODN template with CRISPR/Cas9 technology. This mutation is the equivalent of the human c.5054G>A p.R1684H mutation associated with autism. (J:334099)