A loxP site was inserted upstream of exon 1 and an FRT-flanked PGK-neomycin cassette and second loxP site were inserted downstream of exon 2. The selection cassette was removed via flp-mediated recombination, leaving exons 1-2 floxed. (J:340035)
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A loxP site was inserted upstream of exon 1 and an FRT-flanked PGK-neomycin cassette and second loxP site were inserted downstream of exon 2. The selection cassette was removed via flp-mediated recombination, leaving exons 1-2 floxed. (J:340035)