Syt7^tm3.2Sud

Using homologous recombination, exon 2 of the mouse Syt7 gene that encodes the transmembrane region was modified to introduce a haemagglutinin (HA) tag between the Syt7 protein N-terminal and the transmembrane region; in addition, a loxP site was introduced into the 5 intron and a neomycin resistance cassette (NEO) that was flanked by frt1 sites and was followed by a second loxP site introduced in the 3 intron. Flp recombinase removed the frt flanked neo-selection cassette. Cre recombinase removed the loxP-flanked exon 2. (J:249264)