Log In|Sign Up
EN
The targeting vector was designed to insert the Sirt6-FLAG cDNA into the Gt(ROSA)26Sor driven by its promoter and controlled by a STOP cassette. The STOP cassette in the vector consisted of a splice acceptor and the floxed (PGK)/gb2neoPGKpolyA2xSV40pA sequence and was inserted in intron 1 of ROSA26 locus. Mouse Sirt6 cDNA with a C-terminal FLAG tag was cloned immediately downstream of the STOP cassette. The Sirt6-FLAG sequence was followed by a BGHpA bovine growth hormone polyadenylation (BGHpA) sequence. (J:338782)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
Show/Hide columns
| Phenotypes |
|---|
References Literature
Title
PMID
Journal
Year
IF
