The targeting construct placed a FRT-flanked Neo gene at intron 3 and a pair of loxP sites flank the FRT-flanked Neo and exon 4. The Neo gene was removed by crossing with a Flp mouse line. Cre-mediated recombination removed exon 4. (J:104881)
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The targeting construct placed a FRT-flanked Neo gene at intron 3 and a pair of loxP sites flank the FRT-flanked Neo and exon 4. The Neo gene was removed by crossing with a Flp mouse line. Cre-mediated recombination removed exon 4. (J:104881)