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CRISPR/cas9 genome editing used guide RNAs [CCC CCGGGATGGCTCTGATATA] to insert an GSG-P2A peptide sequence and mVenus fluorescent protein coding sequence into the 3' UTR. Foxi3 transcript Foxi3-201 (ENSMUST00000069634.6) was used as reference for the exon number and guide sequences. Donor DNA contained two nucleotide changes (C to T and C to G) to prevent the inserted sequence from being targeted by the Foxi3 gRNA. (J:339365)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
