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The targeting construct contains the Myf5 open reading frame (ORF), a Kozak sequence to allow protein translation initiation. A T2A self-cleaving peptide, E2Crimson fluorescent reporter sequence, and 3 nuclear localization signals (3xNLS) inserted after exon 3, just upstream of the 3' untranslated region (UTR), of the Myf5 gene. An FRT-flanked puromycin selection cassette was also included after the 3' UTR. Flp-mediated recombination removed the puromycin cassette. (J:339277)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
