Prm1^em1Ssha

CRISPR/Cas9 technology generated an AAA to GCA change resulting in a lusine to alanine substitution at amino acid 49 (p.K49A). A few synonymous alterations were incorporated to introduce an FspI site for genotyping. The K49A mutation results in loss of positive charge as well as loss of acetylation at the site. (J:338968)