Stat1^tm1.1Shwj

A loxP site was inserted into intron 9 and an FRT site flanked neomycin resistance gene cassette, an mloxP site, an inverted and mutated copy of exon 10, a second loxP site and a second mloxP site were inserted into intron 10. Only after Cre-mediated recombination, when the mutated exon 10 inverts and replaces the wildtype exon through specific recombination between the two loxP sites and the two mloxP sites, will the mutated transcript and peptide be expressed. The mutation involved changing arginine codon 274 (CGT) to glutamine (CAA) (p.R274Q), which replicates the same human mutation found in autosomal dominant chronic mucocutaneous candidiasis (CMCC) patients. The neo cassette was removed through subsequent Flp-mediated recombination. (J:338134)