The targeting construct inserted a loxP site upstream of exon 8 and an FRT-flanked neomycin resistance cassette with a 5' loxP site downstream of exon 10. Flp-mediated recombination removed the selection cassette. (J:263975)
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The targeting construct inserted a loxP site upstream of exon 8 and an FRT-flanked neomycin resistance cassette with a 5' loxP site downstream of exon 10. Flp-mediated recombination removed the selection cassette. (J:263975)