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CRISPR/cas9 endonuclease-mediated genome editing is used to create a single base substitution at c.3583-9 (G to A) that creates a cryptic splice acceptor site and premature stop codon in intron 16. The aberrant splicing results in a 7 base pair addition within mRNA transcripts.The mutation was confirmed by sequencing and Southern blot. (J:341855)