Nmu^{tm1.1(cre)Debu}

A targeting construct was designed to insert a Cre coding sequence, an IRES element, the coding sequence of NMU isoform 1, an hGHpolyA signal, and a neomycin cassette for positive selection, flanked by two flippase recognition target (FRT) sites in frame with exon 1 ATG of the Nmu gene on chromosome 5 maintaining the expression of the most prevalent isoform of the Nmu gene products. After homologous recombination in ES cells, resulting chimeras were crossed to Flp deleter mice to excise the neo-cassette. (J:338487)