A loxP site was inserted upstream of exon 1 and an FRT-flanked neomycin resistance cassette and second loxP site were inserted downstream of exon 7. Exons 1-7 and the neomycin were removed via cre-mediated recombination in the germline. (J:337771)
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A loxP site was inserted upstream of exon 1 and an FRT-flanked neomycin resistance cassette and second loxP site were inserted downstream of exon 7. Exons 1-7 and the neomycin were removed via cre-mediated recombination in the germline. (J:337771)