A loxP site was inserted upstream of exon 1 and an FRT-flanked neomycin resistance cassette and second loxP site were inserted downstream of exon 7. The neomycin resistance cassette was removed via flp-mediated recombination. (J:262962)
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A loxP site was inserted upstream of exon 1 and an FRT-flanked neomycin resistance cassette and second loxP site were inserted downstream of exon 7. The neomycin resistance cassette was removed via flp-mediated recombination. (J:262962)