Trem2^em1Npa

CRISPR/Cas9 technology generated a CACAGCACC to ATCCCCGAC change, resulting in the substitution of amino acids histidine 157, serine 158 and threonine 159 (HST) to isoleucine, proline, and aspartate (IPD). IPD are the least-preferred amino acids for the sub-site biding of ADAM10/17 and this change is expected to reduce TREM2 proteolytic cleavage. (J:337819)