A transgene consists of the 2.4 kb mouse Prrx1 enhancer followed by the b-globin intron and nuclear-localized teal fluorescent protein-1 (mTFP1-nls), a T2A self-cleaving peptide, and CreERT2. Six founder animals were generated and a single line with the ability to induce the recombination of LoxP reporter lines only after administration of tamoxifen was established and used for the studies. (J:337818)