A loxP-F3-hygromycin-F3 selection cassette was placed in intron 1 and an FRT-ivNeo-FRT-loxP cassette was positioned 257bp downstream of exon 14. Flp-mediated recombination removed the hygromycin and neomycin cassettes. (J:101977)
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A loxP-F3-hygromycin-F3 selection cassette was placed in intron 1 and an FRT-ivNeo-FRT-loxP cassette was positioned 257bp downstream of exon 14. Flp-mediated recombination removed the hygromycin and neomycin cassettes. (J:101977)