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The first targeting vector is designed to insert loxP sites flanking exon 4 and an FRT-flanked neomycin cassette in reverse orientation downstream of exon 4. In this targeting event the upstream loxP site was lost. A second targeting event used the 129S2/SvPas-derived embryonic stem cells from the first targeting event and placed a LoxP-Hygro-LoxP upstream of exon 3, and generated mice with LoxP-Hygro-LoxP-Exon3 -Exon 4-LoxP. Flp-mediated recombination removed the neo cassette. Cre-mediated recombination removed the hygro cassette. Exons 3 and 4 encode 75% of the protein coding sequence as well as the DNA binding domain. (J:335842)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
