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CRISPR/cas9 methodologies are used to introduce an H170N (nucleotide change CAC to AAT, note AAC was originally reported) in exon 2. The mutation disrupts interactions between FLRT2 and its ligands UNC5C and UNC5D. A single guide RNA (sgRNA) was produced in which the PAM sequence overlaps the H170 codon (sequence 5' GCCCAACAGGCACGCTACTCagg; PAM site is denoted by lowercase letters). The PAM sequence is destroyed by the codon-altering mutation, thereby rendering correctly edited alleles immune to further cas9 cutting activity. (J:339867)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
