Tg(tetO-CAT-Pdx1)1Miya

This Cre recombinase reporter transgene employs the "CAG promoter," which consists of the cytomegalovirus immediate early (CMV-EI) enhancer followed by a 1.3-kb DNA segment including the promoter, first exon and first intron of the chicken beta-actin gene, with the 3' splice junction sequence replaced by that of the rabbit hemoglobin beta gene. The promoter is separated from the PDX1 coding sequence and FLAG epitope tag, with a rabbit globin polyadenylation signal, by a loxP-flanked chloramphenicol acetyltransferase (cat) gene with the SV40 small t intron and polyadenylation signal. (J:127214)