Plasmids encoding a sgRNA (5'- AGGGGAGCACACATTCCACT-3') were designed to fuse a 3xV5 epitope tag and a P2A self-cleaving peptide sequence (P2A)-tdTomato cassette close to the stop codon. Insertion of this reporter cassette does not affect endogenous gene expression. (J:337553)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count