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The allele was generated in the TT2-derived ES cell clone 71-5F2-23 by targeted replacement via RMCE in unknown Igs54 (integrated site) in the mouse locus. The original ES cell clone integrated the CAG-lox71-bsr-pA-lox511-FRT-lox2272 target lox sites. The bsr gene was replaced with the lox66-cre-pA-MC1neopA-lox2272 expression cassette through Cre-mediated recombination so that the ubiquitous CAG promoter drives Cre recombinase expression. After RMCE, ES cell clone line e was selected and generated chimera, and germline transmission was confirmed. (J:243719)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
