Mocs2^tm2Geno

The Mocs2B subunit mutation, a 2 bp deletion of AA at position 726 and 727 (c.726_727delAA) was introduced via homologous recombination. The deletion of the two nucleotides is predicted to result in the replacement of the last nine amino acids by seven de novo amino acids in the mouse. In the human, the same deletion results in the addition of 30 de novo amino acids. To resemble the pathological state in humans, the last 27 coding nucleotides in the mouse sequence was replaced by the human sequence coding for the 30 de novo amino acid as expected for the c.726_727delAA mutation. A loxP-flanked neomycin cassette was inserted upstream of exon 8. (J:336726)