Foxi3^em1Ybz

CRISPR/Cas9 technology using gRNA TCGGAAGCGAAGGCGCCGG-CGG was used to generate mice carrying three variants: an arginine to tryptophan at residue 220 (codon from CGG to TGG), an arginine to glutamine at residue 222 (codon from CGA to CAA), and an arginine to histidine at residue 224 (codon from CGC to CAC) (p.R220W, p.R222Q, and p.R224H). These are orthologous to p.R236W, p.R238Q, and p.R240H pathogenic variants identified in Chinese families with Goldenhar syndrome (craniofacial microsomia). (J:336768)