Gt(ROSA)26Sor^{tm1(CAG-Gria2*)Bli}

The targeting cassette includes (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), an FRT site, a loxP-flanked STOP cassette (with stop codons in all 3 reading frames and a triple polyA signal), Gria2 mutant cDNA (containing two point mutations, L483Y and R845A), a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), a polyA signal, and an attB/attP-flanked PGK-FRT-Neo-polyA cassette. This targeting vector was inserted into intron 1 of the Gt(ROSA)26Sor locus. The L483Y mutation impairs AMPA receptor desensitization and the R845A mutation blocks AMPA receptor endocytosis. (J:262751)