Two loxP sites flanking a STOP cassette sequence and a frt-flanked PKG-neomycin cassette were inserted into intron 3. The frt-flanked PKG-neomycin cassette was removed via FLP-mediated recombination in the germline. (J:297217)
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Two loxP sites flanking a STOP cassette sequence and a frt-flanked PKG-neomycin cassette were inserted into intron 3. The frt-flanked PKG-neomycin cassette was removed via FLP-mediated recombination in the germline. (J:297217)