A FRT-flanked puromycin cassette containing a loxP sequence was inserted 5' to exon 4 and a single loxP site was inserted 5' to exon 5. The FRT-flanked puromycin cassette was removed via FLP-mediated recombination in the germline. (J:248803)
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A FRT-flanked puromycin cassette containing a loxP sequence was inserted 5' to exon 4 and a single loxP site was inserted 5' to exon 5. The FRT-flanked puromycin cassette was removed via FLP-mediated recombination in the germline. (J:248803)