Log In|Sign Up
EN
Using the CRISPR/Cas9 method, a targeting construct consisting of an AS-loxP/NeoSTOP/loxP-murine Mecom cDNA followed by IRES-EGFP-pA fusion gene inserted into intron 1 of the ROSA26 locus into STOP-eGFP-ROSA26TV plasmid was injected into 6NK7 embryonic stem cells. Mecom and EGFP are expressed after recombination by Cre recombinase driven by endogenous Gt(ROSA)26Sor promoter. The lines containing this allele showed a low recombination rate. (J:336140)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
Show/Hide columns
| Phenotypes |
|---|
References Literature
Title
PMID
Journal
Year
IF
