The LoxP-Stop-LoxP-IRES-TeNT (tetanus toxin light chain) gene cassette was knocked into the 3'-noncoding region of the gene via homologous recombination. Flp-mediated recombination removed the selection cassette. (J:210615)
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The LoxP-Stop-LoxP-IRES-TeNT (tetanus toxin light chain) gene cassette was knocked into the 3'-noncoding region of the gene via homologous recombination. Flp-mediated recombination removed the selection cassette. (J:210615)