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Arginine codon 140 (CGT) in exon 2 was targeted for change to histidine (CAT) (c.419G>A p.R140H) using an sgRNA (targeting AACGCACTGCGTAGTTGAGT) and an ssODN template with CRISPR/Cas9 technology. This allele represents an incompletely repaired sequence with a 4 bp deletion around the intended mutation (c.417_420delGCGT) that introduces a frameshift and a premature stop codon shortly thereafter (p.R140Wfs*10) and is thus a knock-out allele. (J:325410)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
