Ret^em2Heno

Tyrosine codon 792 (TAT) was changed to phenylalanine (TTT) (p.Y792F) using an sgRNA (targeting ACATGTCATCAAGTTGTATGGGG) and an ssODN template with CRISPR/Cas9 technology. This mutation is the equivalent of the human p.Y791F mutation associated with Hirschsprung disease (HSCR). (J:325770)