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The targeting construct inserted an IRES sequence followed by the mER:Cre:mER cDNA encoding a fusion protein with two copies of the mouse estrogen receptor (mER) flanking Cre recombinase, and a Neomycin resistance (NeoR) selection cassette flanked by Frt recombination sites into an Eag1 site in the Fgf8 gene located 36 bp 3 of the Fgf8 stop codon and 5 to the endogenous polyadenylation sequence. The linearized targeting vector was electroporated into mouse embryonic stem cells and subjected to selection. Flp-recombinase excised the NeoR. (J:301046)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
