Gt(ROSA)26Sor^tm8Sor

A viral splice acceptor (SA)-loxP-neomycin-pA-loxP-FOXO1-mRuby3-P2A-H2B-mTagBFP2 (BFP)-P2A-ERK-KTR-mClover3-WPRE-pA cassette is introduced to the mouse Gt(ROSA)26Sor locus. The targeting vectorincludes DNA fragments contained mClover3 (Addgene, #(a href="https://www.addgene.org/74252/" target="_blank")74252(/a)), mRuby3 (Addgene, #(a href="https://www.addgene.org/74252/" target="_blank")74252(/a)), mTagBFP2 (Addgene, #(a href="https://www.addgene.org/99267/" target="_blank")99267(/a); BFP), human H2B (Addgene, #(a href="https://www.addgene.org/99267/" target="_blank")99267(/a)), an adenovirus splice acceptor, a floxed NeoR sequence, the ERK-KTR reporter, the FOXO1 reporter, and woodchuck hepatitis virus post-transcriptional regulatory element (from ROSA26(Tdt) mouse genomic DNA. The ERK-KTR sensor consists of an ERK1/2 binding site and phosphorylation motif adjacent to phosphorylation activated nuclear export signal (NES) and inhibited nuclear localization sequence (NLS) fused to a fluorescent protein (FP; mClover3). The AKT sensor uses a mutant version of FOXO1 with defective DNA binding and relies on endogenous AKT phosphorylation sites that regulate its nuclear localization. (J:339772)