Gt(ROSA)26Sor^{em1.1(CAG-EGFP/Rpl10)Mgmj}

The allele is generateb from Gt(ROSA)26Sorem1(CAG-EGFP/Rpl10)Mgmj by germline deletion of the lox-stop-lox cassette. Using CRISPR/Cas9 technology, a targeting vector CAG-FSF-LSL followed by an EGFP/Rpl10 fusion reporter gene under the control of the CAG promoter (CVM enhancer/chicken ACTB promoter) was inserted into the Gt(ROSA)26Sor locus. The lox-stop-lox cassette (LSL) was removed by crossing to a germ line cre-deleter. It is an Flp-dependent reporter. The expression of the EGFP/Rpl10 fusion reporter gene requires the removal of FSF by Flp recombinases. (J:317381)