The first 2 exons were floxed and and FRT flanked neo cassette was inserted in intron 2 via homologous recombination. Flp mediated recombination removed the neo cassette. (J:309142)
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The first 2 exons were floxed and and FRT flanked neo cassette was inserted in intron 2 via homologous recombination. Flp mediated recombination removed the neo cassette. (J:309142)