Exon 1 was flanked by loxP sites and an FRT-flanked neomycin cassette was inserted downstream of the floxed exon. The neomycin cassette was removed via flp-mediated recombination. Exon 1 was deleted via flp-mediated recombination. (J:236346)
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Exon 1 was flanked by loxP sites and an FRT-flanked neomycin cassette was inserted downstream of the floxed exon. The neomycin cassette was removed via flp-mediated recombination. Exon 1 was deleted via flp-mediated recombination. (J:236346)