The transgene insert contains the following elements: 5.8 kb sequence containing Nr5a1 promoter and gene sequence up to and including 5' UTR on exon 2, the lacZ reporter gene, SV40 ply(A) signal sequences and 502 bp sequence from Nr5a1 intron 6 containing a ventromedial hypothalamus-specific (VMH)-specific Nr5a1 enhancer. (J:334341)