Rr253^em6Kmm

Using zygotes that already carry the Rr253em4Kmm NFIL3C/EBP binding site 1&2-deletion allele, binding site 3 in the Zeb2 enhancer, located ~165 kb upstream, were targeted with an sgRNA (targeting CCACCAGCCGGTGACACACA) and an ssODN template (GCTGAAGTTCACCTCCCCATGGGGGAATGAATCATCAAAATAACCGGTGGAAAACAAGCTAGATCTGACTTGAGCAGTGTGTGTCACCGGCTGGTGGAATTTTTAGAAAGGCAGCAGTTTGGGCTCATCACTGCGGTT) using CRISPR/Cas9 technology, resulting in an allele lacking binding sites 1, 2 and 3. (J:334296)